	US 11,834,665 B2
	Programmable transcription factors and methods
Michael J. Smanski, Minneapolis, MN (US); and Maciej Maselko, Minneapolis, MN (US)
Assigned to REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US)
Appl. No. 16/611,008
Filed by REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US)
PCT Filed May 10, 2018, PCT No. PCT/US2018/031950 § 371(c)(1), (2) Date Nov. 5, 2019, PCT Pub. No. WO2018/209014, PCT Pub. Date Nov. 15, 2018.
Claims priority of provisional application 62/504,260, filed on May 10, 2017.
Prior Publication US 2020/0157552 A1, May 21, 2020
Int. Cl. C12N 15/63 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01)

CPC C12N 15/635 (2013.01) [C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01); C12N 2830/002 (2013.01); C12N 2830/005 (2013.01)]

12 Claims

1. A cell comprising a programmable transcription activator system, the system comprising:

a programmable transcription activator comprising:

a domain that specifically binds to a promoter; and

a transcriptional activator domain;

a polynucleotide comprising a coding region whose expression is required for cell viability of the cell;

a promoter operably linked to the polynucleotide, the promoter comprising a binding site for the programmable transcription activator; and

a non-essential coding region operably linked to the promoter.