US 11,834,650 B1
Methods of transfection using sonoporation
Anne Ruffing, Albuquerque, NM (US); Darren W. Branch, Albuquerque, NM (US); and Ronald P. Manginell, Albuquerque, NM (US)
Assigned to National Technology & Engineering Solutions of Sandia, LLC, Albuquerque, NM (US)
Filed by National Technology & Engineering Solutions of Sandia, LLC, Albuquerque, NM (US)
Filed on Jun. 20, 2019, as Appl. No. 16/446,971.
Claims priority of provisional application 62/689,003, filed on Jun. 22, 2018.
Int. Cl. C12N 15/87 (2006.01); C12N 13/00 (2006.01); C12M 1/42 (2006.01)
CPC C12N 13/00 (2013.01) [C12M 35/04 (2013.01); C12N 15/87 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method of transforming a cell, the method comprising:
introducing a cell and an agent into at least one channel of an acoustic cell system comprising an acoustic transducer, wherein the acoustic transducer is configured to be disposed beneath the at least one channel and is adapted to propagate an acoustic wave in the at least one channel;
providing a radiofrequency signal to the acoustic transducer, thereby propagating the acoustic wave and generating localized acoustic pressure in proximity to the cell; and
delivering the agent into the cell, thereby transforming the cell;
wherein the acoustic cell system comprises a surface acoustic wave system or a bulk acoustic wave system;
wherein the cell comprises an algal cell;
wherein the introducing step is conducted in the presence of a nutrient medium;
wherein the nutrient medium has a salinity of from about 3% to about 30%.