	US 11,834,643 B2
	Continuous perfusion bioreactor system
Parrish M. Galliher, Littleton, MA (US); Geoffrey L. Hodge, Sutton, MA (US); Michael Fisher, Ashland, MA (US); Patrick Guertin, Mendon, MA (US); and Dan Mardirosian, Charlton, MA (US)
Assigned to GLOBAL LIFE SCIENCES USA LLC, Marlborough, MA (US)
Filed by GLOBAL LIFE SCIENCES SOLUTIONS USA LLC, Marlborough, MA (US)
Filed on Aug. 5, 2021, as Appl. No. 17/394,864.
Application 14/743,960 is a division of application No. 12/124,039, filed on May 20, 2008, granted, now 9,109,193.
Application 17/394,864 is a continuation of application No. 14/743,960, filed on Jun. 18, 2015, granted, now 11,111,471.
Claims priority of provisional application 60/962,671, filed on Jul. 30, 2007.
Prior Publication US 2021/0371793 A1, Dec. 2, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/00 (2006.01); C12M 3/00 (2006.01); C12M 1/06 (2006.01); B01D 29/13 (2006.01)

CPC C12M 29/10 (2013.01) [B01D 29/13 (2013.01); C12M 23/14 (2013.01); C12M 23/26 (2013.01); C12M 23/48 (2013.01); C12M 27/02 (2013.01); C12M 27/20 (2013.01); C12M 29/00 (2013.01); C12M 29/18 (2013.01); C12M 47/10 (2013.01)]

22 Claims

1. A bioreactor apparatus, comprising:

a first reusable support structure with walls configured to support a first collapsible bag containing a solids-bearing liquid, the first collapsible bag containing at least a first inlet and a first outlet;

a second reusable support structure with walls configured to support a second collapsible bag fluidically coupled via a first fluid connection from the first outlet of the first collapsible bag to a first inlet of the second collapsible bag;

wherein the first fluid connection is accomplished through the walls of the first and second reusable support structures; and

wherein the second collapsible bag is configured to separate solids from the solids-bearing liquid received from the first collapsible bag and return a solids-separated liquid to the first collapsible bag via the first inlet of the first collapsible bag.