CPC B01L 3/502761 (2013.01) [B01L 3/502715 (2013.01); C12N 15/1003 (2013.01); C12Q 1/02 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0854 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/0864 (2013.01)] | 15 Claims |
1. A method of obtaining purified viable uninucleate microspores by screening plant cells using fluorescence lifetime imaging microscopy (FLIM), the method comprising:
sorting a population of plant cells by size exclusion, said population of plant cells comprising microspores in any developmental stage, tetrads or pollen grain cells to obtain a first population of purified uninucleate microspores;
imaging the first population of purified uninucleate microspores using FLIM;
selecting a second population of purified viable uninucleate microspores from the first population of purified uninucleate microspores based on the FLIM imaging; and
obtaining the second population of purified viable uninucleate microspores.
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