US 11,833,504 B2
Microfluidic label-free isolation and identification of cells using fluorescence lifetime imaging (FLIM)
Abraham P. Lee, Irvine, CA (US); Michelle A. Digman, Irvine, CA (US); Dohyun Lee, Irvine, CA (US); Xuan Li, Irvine, CA (US); Ning Ma, Irvine, CA (US); and Yue Yun, Johnston, IA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US); and PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US); and PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed on Apr. 13, 2020, as Appl. No. 16/847,305.
Application 16/847,305 is a continuation in part of application No. PCT/US2018/055722, filed on Oct. 12, 2018.
Claims priority of provisional application 62/571,746, filed on Oct. 12, 2017.
Prior Publication US 2020/0238288 A1, Jul. 30, 2020
Int. Cl. B01L 3/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/02 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/502715 (2013.01); C12N 15/1003 (2013.01); C12Q 1/02 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0854 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/0864 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method of obtaining purified viable uninucleate microspores by screening plant cells using fluorescence lifetime imaging microscopy (FLIM), the method comprising:
sorting a population of plant cells by size exclusion, said population of plant cells comprising microspores in any developmental stage, tetrads or pollen grain cells to obtain a first population of purified uninucleate microspores;
imaging the first population of purified uninucleate microspores using FLIM;
selecting a second population of purified viable uninucleate microspores from the first population of purified uninucleate microspores based on the FLIM imaging; and
obtaining the second population of purified viable uninucleate microspores.