US 11,833,503 B2
Methods and compositions for surface functionalization of optical semiconductor-integrated biochips
Andrea Cuppoletti, Livermore, CA (US); Arjang Hassibi, Santa Clara, CA (US); Lei Pei, San Jose, CA (US); Yang Liu, San Jose, CA (US); Kshama Jirage, Palo Alto, CA (US); and Arun Manickam, San Jose, CA (US)
Assigned to InSilixa, Inc., Sunnyvale, CA (US)
Filed by InSilixa, Inc., Sunnyvale, CA (US)
Filed on Dec. 7, 2021, as Appl. No. 17/544,776.
Prior Publication US 2023/0173483 A1, Jun. 8, 2023
Int. Cl. B01L 3/00 (2006.01); H01L 27/146 (2006.01); C23C 16/02 (2006.01); C07F 7/08 (2006.01); B01L 7/00 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 3/502707 (2013.01); B01L 3/502738 (2013.01); B01L 7/52 (2013.01); C07F 7/089 (2013.01); C23C 16/0272 (2013.01); H01L 27/14645 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/06 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for immobilizing a nucleic acid construct to a surface of a substrate, the method comprising:
immobilizing a silane to the surface of the substrate, wherein the silane comprises a first chemical group; and
contacting the silane with the nucleic acid construct comprising a second chemical group, wherein the contacting is performed for less than 10 minutes;
wherein the substrate comprises an integrated optical sensor circuit comprising a complementary metal-oxide-semiconductor (CMOS) integrated circuit (IC), wherein (1) the first chemical group comprises a first alkyne and the second chemical group comprise a first azide; or (2) the first chemical group comprises a second azide and the second chemical group comprises a second alkyne, wherein the first and second alkyne is a dibenzocyclooctyne (DBCO), or a derivative thereof, wherein the first chemical group reacts with the second chemical group via a strain-promoted alkyne-azide cycloaddition (SPAAC), thereby immobilizing the nucleic acid construct on the surface of the substrate.