US 11,832,601 B2
Cytoplasmic incompatibility factors and methods for controlling anthropods
Jason Metcalf, Dexter, MI (US); Seth R. Bordenstein, Nashville, TN (US); Daniel Lepage, Centertown, MO (US); Sarah Bordenstein, Nashville, TN (US); Mark Hochstrasser, Hamden, CT (US); John F. Beckmann, New Haven, CT (US); and Judith Ronau, Hamden, CT (US)
Assigned to VANDERBILT UNIVERSITY, Nashville, TN (US); and YALE UNIVERSITY, New Haven, CT (US)
Appl. No. 16/307,982
Filed by VANDERBILT UNIVERSITY, Nashville, TN (US); and YALE UNIVERSITY, New Haven, CT (US)
PCT Filed Jun. 9, 2017, PCT No. PCT/US2017/036693
§ 371(c)(1), (2) Date Dec. 7, 2018,
PCT Pub. No. WO2017/214476, PCT Pub. Date Dec. 14, 2017.
Claims priority of provisional application 62/347,818, filed on Jun. 9, 2016.
Prior Publication US 2019/0216064 A1, Jul. 18, 2019
Int. Cl. A01K 67/033 (2006.01); C12N 15/87 (2006.01); C12N 1/20 (2006.01); C07K 14/195 (2006.01); C12N 9/22 (2006.01); C12N 9/64 (2006.01); C12R 1/01 (2006.01)
CPC A01K 67/0337 (2013.01) [A01K 67/0339 (2013.01); C07K 14/195 (2013.01); C12N 1/205 (2021.05); C12N 9/22 (2013.01); C12N 9/6472 (2013.01); C12N 15/87 (2013.01); A01K 2217/052 (2013.01); A01K 2217/206 (2013.01); A01K 2227/706 (2013.01); A01K 2267/02 (2013.01); C07K 2319/21 (2013.01); C12R 2001/01 (2021.05)] 4 Claims
 
1. A genetically modified arthropod, said arthropod comprising:
(i) a bacterial operon or two independent chromosomal insertions encoding at least two cytoplasmic incompatibility factors, wherein the bacterial operon or the two independent chromosomal insertions is from Wolbachia;
wherein the bacterial operon or the two independent chromosomal insertions encode: the cytoplasmic incompatibility factors WD0631 and WD0632; and
wherein WD0631 is encoded by SEQ ID NO: 25; and
(ii) a promoter operably linked to the bacterial operon or the two independent chromosomal insertions;
wherein the expression of the cytoplasmic incompatibility factors in a male arthropod causes a reduction in viable offspring in comparison to a male arthropod lacking the cytoplasmic incompatibility factors.