US 10,138,502 B2
Method for producing oil containing polyunsaturated fatty acid using lipase
Hideo Ikemoto, Hachioji (JP); Nobushige Doisaki, Hachioji (JP); and Yasuo Umehara, Hakodate (JP)
Assigned to NIPPON SUISAN KAISHA, LTD., Tokyo (JP)
Filed by NIPPON SUISAN KAISHA, LTD., Chiyoda-ku, Tokyo (JP)
Filed on Apr. 15, 2016, as Appl. No. 15/99,899.
Application 15/099,899 is a continuation of application No. 13/700,647, abandoned, previously published as PCT/JP2011/062170, filed on May 27, 2011.
Claims priority of application No. 2010-122688 (JP), filed on May 28, 2010.
Prior Publication US 2016/0230199 A1, Aug. 11, 2016
Int. Cl. C12P 7/64 (2006.01); A23D 9/04 (2006.01); A23D 9/013 (2006.01); C11B 3/00 (2006.01); C11B 3/16 (2006.01); C11C 1/04 (2006.01)
CPC C12P 7/6472 (2013.01) [A23D 9/013 (2013.01); A23D 9/04 (2013.01); C11B 3/001 (2013.01); C11B 3/003 (2013.01); C11B 3/16 (2013.01); C11C 1/045 (2013.01); Y02E 50/17 (2013.01)] 14 Claims
 
1. A method for lowering saturated fatty acid content in a glyceride containing a polyunsaturated fatty acid, the method comprising:
concentrating the polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to hydrolyze the glyceride containing the polyunsaturated fatty acid, wherein the glyceride containing the polyunsaturated fatty acid is a marine animal oil glyceride or a microorganism oil glyceride;
wherein the polyunsaturated fatty acid is a fatty acid having at least 20 carbon atoms and having at least 3 double bonds,
wherein the hydrolysis by the lipase is performed in the presence of 10 to 200 weight percent of water based on a total weight of the glyceride containing the polyunsaturated fatty acid, and at a temperature from 10° C. to 20° C.,
wherein the lipase is derived from a microorganism selected from the group consisting of Candida cylindracea and Alcaligenes sp.; and
wherein the saturated fatty acid content in the resulting glyceride is less than the saturated fatty acid content in the glyceride prior to the concentrating.