CPC G01N 33/533 (2013.01) [G01N 33/558 (2013.01); G01N 33/577 (2013.01)] | 10 Claims |
1. A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol, and T-2 toxin, comprising an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of an europium-labeled anti-4,15-diacetoxyscirpenol monoclonal antibody, an europium-labeled anti-deoxynivalenol monoclonal antibody and an europium-labeled anti-T-2 toxin monoclonal antibody, wherein the immunochromatography time-resolved fluorescence test strip comprises a liner, wherein a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and three detection lines are arranged on the nitrocellulose membrane from top to bottom, the transverse quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the transverse quality control line, and the detection lines are coated with a 4,15-diacetoxyscirpenol-bovine serum albumin conjugate, a deoxynivalenol-bovine serum albumin conjugate and a T-2 toxin-ovalbumin conjugate respectively; and the anti-4,15-diacetoxyscirpenol monoclonal antibody is secreted by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO:C201881.
|