US 11,815,511 B2
Time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol, and T-2 toxin
Qi Zhang, Hubei (CN); Xiaoqian Tang, Hubei (CN); Yizhen Bai, Hubei (CN); Wen Zhang, Hubei (CN); and Peiwu Li, Hubei (CN)
Assigned to OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES, Hubei (CN)
Appl. No. 17/777,268
Filed by OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES, Hubei (CN)
PCT Filed Nov. 16, 2020, PCT No. PCT/CN2020/129074
§ 371(c)(1), (2) Date May 16, 2022,
PCT Pub. No. WO2021/093886, PCT Pub. Date May 20, 2021.
Claims priority of application No. 201911122507.3 (CN), filed on Nov. 15, 2019.
Prior Publication US 2022/0412965 A1, Dec. 29, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/533 (2006.01); G01N 33/558 (2006.01); G01N 33/577 (2006.01)
CPC G01N 33/533 (2013.01) [G01N 33/558 (2013.01); G01N 33/577 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol, and T-2 toxin, comprising an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of an europium-labeled anti-4,15-diacetoxyscirpenol monoclonal antibody, an europium-labeled anti-deoxynivalenol monoclonal antibody and an europium-labeled anti-T-2 toxin monoclonal antibody, wherein the immunochromatography time-resolved fluorescence test strip comprises a liner, wherein a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and three detection lines are arranged on the nitrocellulose membrane from top to bottom, the transverse quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the transverse quality control line, and the detection lines are coated with a 4,15-diacetoxyscirpenol-bovine serum albumin conjugate, a deoxynivalenol-bovine serum albumin conjugate and a T-2 toxin-ovalbumin conjugate respectively; and the anti-4,15-diacetoxyscirpenol monoclonal antibody is secreted by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO:C201881.