CPC C12Q 1/6874 (2013.01) [C12Q 1/6869 (2013.01); G16B 25/00 (2019.02); G16B 30/00 (2019.02); G16B 30/10 (2019.02); G16B 25/20 (2019.02)] | 20 Claims |
1. A method for processing target nucleic acids derived from a plurality of samples, comprising:
(a) contacting a plurality of index polynucleotides with target nucleic acids derived from the plurality of samples to generate a plurality of index-target polynucleotides, wherein:
index polynucleotides contacted with target nucleic acids derived from each sample comprise an index sequence or a combination of index sequences that is uniquely associated with that sample,
the index sequence or the combination of index sequences is selected from a set of index sequences, and
the set of index sequences comprises a plurality of pairs of color-balanced index sequences, wherein any two bases at corresponding sequence positions of each pair of color-balanced index sequences include both (i) an adenine (A) base or a cytosine (C) base, and (ii) a guanine (G) base, a thymine (T) base, or a uracil (U) base.
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