CPC C12Q 1/6813 (2013.01) [C12Q 2531/119 (2013.01); C12Q 2537/137 (2013.01); C12Q 2537/1373 (2013.01); C12Q 2600/178 (2013.01)] | 11 Claims |
1. A method for detecting a target nucleic acid comprising:
(a) mixing a molecular beacon containing locked nucleic acid (LNA) and a sample containing the target nucleic acid to form a composition, wherein the molecular beacon containing LNA is a double-stranded nucleic acid complex comprising a first single strand comprising a toehold region complementary to the target nucleic acid and a fluorescent reagent, and a second single strand bound with graphene oxide, wherein the first single strand and the second single strand are complementary to each other at least in part;
(b) complementarily binding the toehold region of the first single strand to the target nucleic acid and displacing the second single strand through toehold-mediated strand displacement; and
(c) detecting a fluorescence signal of the fluorescent reagent, thereby detecting the target nucleic acid.
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