	US 11,814,670 B2
	Modification of DNA on magnetic beads
Michael J. Domanico, Middleton, WI (US); Hatim Allawi, Middleton, WI (US); Graham P. Lidgard, Madison, WI (US); Brian Aizenstein, Madison, WI (US); Oliver Hunt, Madison, WI (US); and Tobias Charles Zutz, Madison, WI (US)
Assigned to Exact Sciences Corporation, Madison, WI (US)
Filed by Exact Sciences Corporation, Madison, WI (US)
Filed on May 29, 2020, as Appl. No. 16/888,397.
Application 16/888,397 is a continuation of application No. 16/164,373, filed on Oct. 18, 2018, granted, now 10,704,083.
Application 16/164,373 is a continuation of application No. 15/068,121, filed on Mar. 11, 2016, granted, now 10,144,953, issued on Dec. 4, 2018.
Application 15/068,121 is a continuation of application No. 13/754,631, filed on Jan. 30, 2013, granted, now 9,315,853, issued on Apr. 19, 2016.
Claims priority of provisional application 61/592,272, filed on Jan. 30, 2012.
Prior Publication US 2020/0291459 A1, Sep. 17, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6806 (2018.01); C12Q 1/6851 (2018.01)

CPC C12Q 1/6806 (2013.01) [C12Q 1/6851 (2013.01); C12Q 2600/154 (2013.01)]

13 Claims

1. A system comprising:

a) a composition comprising:

i) single-stranded small DNA comprising sulfonated cytosine deoxynucleotides, wherein the small DNA is 200 or fewer nucleotides in length, and wherein the small DNA is bound to silica or silica-coated beads, in a mixture with

ii) an alcohol-free solution comprising ammonium hydrogen sulfite and 5.5 to 7.5 M guanidine hydrochloride;

b) a desulfonation reagent comprising NaOH and isopropanol; and

c) a wash buffer comprising ethanol.