	US 11,814,665 B2
	Enhanced production of lipids by limitation of at least two limiting nutrient sources
Adam Burja, Potomac, MD (US); James Corona, Lutherville, MD (US); Jose R. Garcia, Lorton, VA (US); Goncalo Oliveira Maia, Columbia, MD (US); Horst Priefert, Ostbevern (DE); Joachim Windau, Warendorf (DE); and Gabriel Závodský, Banska Bystrica (SK)
Assigned to Evonik Operations GmbH, Essen (DE); and DSM IP Assets B.V., Te Heerlen (NL)
Appl. No. 16/639,529
Filed by EVONIK OPERATIONS GMBH, Essen (DE); and DSM IP ASSETS B.V., TE Heerlen (NL)
PCT Filed Jul. 18, 2018, PCT No. PCT/EP2018/069454 § 371(c)(1), (2) Date Feb. 14, 2020, PCT Pub. No. WO2019/034354, PCT Pub. Date Feb. 21, 2019.
Claims priority of provisional application 62/546,808, filed on Aug. 17, 2017.
Claims priority of application No. 17192347 (EP), filed on Sep. 21, 2017.
Prior Publication US 2021/0171991 A1, Jun. 10, 2021
Int. Cl. C12P 7/6472 (2022.01); C11B 1/02 (2006.01); C12N 1/38 (2006.01)

CPC C12P 7/6472 (2013.01) [C11B 1/025 (2013.01); C12N 1/38 (2013.01)]

17 Claims

1. A method of producing lipids comprising polyunsaturated fatty acids by the fermentation of a microorganism of the genus Schizochytrium or the genus Aurantiochytrium, said fermentation comprising:

a) a biomass concentration increasing phase, in which a carbon source and at least two limiting nutrient sources are added to fermentation medium containing said microorganism to increase the biomass concentration of said fermentation medium to at least 50 g/L; and

b) a lipid production phase in which the addition of at least two of said limiting nutrient sources is stopped or reduced while supply of the carbon source is maintained;

wherein one of the two limiting nutrient sources is a source of phosphate and the other is a source of nitrogen.