US 11,814,628 B2
Composition and method of an orally administered antimicrobial peptide vectored in a bacterial expression vehicle
Jolieke Gerdy Van Oosterwijk, Memphis, TN (US); Luciana Meirelles Richer, Memphis, TN (US); Douglas Steven Zatechka, Cordova, TN (US); Woohyun Kim, Ellicott city, MD (US); Hyun Soon Lillehoj, West Friendship, MD (US); Cyril Gray, Bethesda, MD (US); and Christopher Anthony Przybyszewski, Southhaven, MS (US)
Assigned to US Biologic, Inc, Memphis, TN (US)
Appl. No. 18/008,512
Filed by US Biologic, Inc., Memphis, TN (US); and The United States of America as represented by the Secretary of Agriculture, Washington, DC (US)
PCT Filed Jun. 14, 2021, PCT No. PCT/US2021/037190
§ 371(c)(1), (2) Date Dec. 6, 2022,
PCT Pub. No. WO2021/257434, PCT Pub. Date Dec. 23, 2021.
Claims priority of provisional application 63/188,894, filed on May 14, 2021.
Claims priority of provisional application 63/040,718, filed on Jun. 18, 2020.
Prior Publication US 2023/0272407 A1, Aug. 31, 2023
Int. Cl. C12N 15/75 (2006.01); A61K 9/00 (2006.01); A61K 35/742 (2015.01); C07K 14/465 (2006.01)
CPC C12N 15/75 (2013.01) [A61K 9/0053 (2013.01); A61K 35/742 (2013.01); C07K 14/465 (2013.01)] 2 Claims
 
1. A method to treat a subject in need of a treatment comprising:
orally administering a therapeutically effective amount of an antimicrobial peptide vectored composition comprising a bacterial protein expression vehicle comprised of a whole cell Bacillus subtilis transformed with a replicable plasmid DNA expression construct codon-optimized for expressing functionally active NK-2, wherein the subject is poultry and said poultry are in need of treatment for Avian coccidiosis, comprising the step of orally administering to the subject the therapeutically effective amount of the antimicrobial peptide vectored composition to induce cytotoxicity to treat an Avian coccidiosis infection.