CPC C07K 14/70539 (2013.01) [B01J 19/0046 (2013.01); C40B 30/04 (2013.01); B01J 2219/00725 (2013.01)] | 19 Claims |
1. A method of a making peptide deficient-MHC class I/chaperone complex, the method comprising:
a) incubating an MHC class I heavy chain, an MHC class I light chain and a placeholder peptide under conditions wherein the MHC class I heavy chain, the MHC class I light chain and the placeholder peptide form a placeholder peptide-MHC class I (p*MHC I) complex; and
b) contacting the placeholder peptide-MHC class I complex with a dipeptide and Tapasin Binding Protein Related (TAPBPR) chaperone, thereby displacing the placeholder peptide from the placeholder peptide-MHC complex and forming the peptide deficient-MHC class I/chaperone complex.
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