US 11,813,604 B2
Printed biogel nanosensors
Christine McBeth, Everett, MA (US); Kirsten Borchers, Stuttgart (DE); Achim Weber, Altbach (DE); and Daniel Zontar, Aachen (DE)
Assigned to Trustees of Boston University, Boston, MA (US); Fraunhofer USA, Inc., Plymouth, MI (US); and FRAUNHOFER-Gesellschaft zur Foerderung der angewandten Forschung e. V., Munich (DE)
Filed by Trustees of Boston University, Boston, MA (US); and Fraunhofer USA, Inc., Plymouth, MI (US)
Filed on May 9, 2022, as Appl. No. 17/740,014.
Claims priority of provisional application 63/186,385, filed on May 10, 2021.
Prior Publication US 2022/0355302 A1, Nov. 10, 2022
Int. Cl. B01L 3/00 (2006.01); C12Q 1/6837 (2018.01); B01L 7/00 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/6876 (2018.01)
CPC B01L 3/5027 (2013.01) [C12Q 1/6837 (2013.01); B01J 2219/00529 (2013.01); B01J 2219/00576 (2013.01); B01J 2219/00626 (2013.01); B01L 7/52 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/6876 (2013.01); C12Q 2565/60 (2013.01); C12Q 2565/607 (2013.01)] 20 Claims
OG exemplary drawing
 
13. A method of preparing a biogel nanosensor comprising:
a) obtaining an acryloyl hydrogel or a methacryloyl hydrogel;
b) printing the hydrogel in picoliter or nanoliter volume on the surface of a substrate in a microarray in the form of spots;
c) crosslinking the hydrogel on the surface of the substrate; and
d) combining nucleic acid amplification reagents in picoliter or nanoliter volume to the cross-linked hydrogel.