US 11,809,792 B2
Model for fluid and mass transport in a recirculating microfluidic system
James Hickman, Orlando, FL (US); Kazi Tasneem, Orlando, FL (US); and Christopher Long, Oviedo, FL (US)
Assigned to University of Central Florida Research Foundation, Orlando, FL (US)
Filed by University of Central Florida Research Foundation, Inc., Orlando, FL (US)
Filed on Sep. 9, 2022, as Appl. No. 17/941,445.
Application 17/941,445 is a continuation of application No. 16/381,660, filed on Apr. 11, 2019, granted, now 11,443,085.
Claims priority of provisional application 62/655,909, filed on Apr. 11, 2018.
Prior Publication US 2023/0043421 A1, Feb. 9, 2023
Int. Cl. G06F 30/23 (2020.01); B01L 3/00 (2006.01); G06F 111/20 (2020.01)
CPC G06F 30/23 (2020.01) [B01L 3/5023 (2013.01); B01L 3/5027 (2013.01); C12Q 2565/629 (2013.01); G06F 2111/20 (2020.01)] 7 Claims
OG exemplary drawing
 
1. A method of performing an experiment using a microfluidic device with recirculation, the microfluidic device comprising a fluid, a first reservoir, a second reservoir in fluid communication with the first reservoir, at least one chamber in fluid communication with the first and second reservoirs, and a fluid path fluidically connecting the first reservoir, the chamber, and the second reservoir, the method comprising:
setting a desired value for a condition within the microfluidic device,
performing a calculation that accounts for an effective pressure difference between the first reservoir and the second reservoir and reactions occurring within the microfluidic device wherein the effective pressure difference is based on a time dependent imposed pressure difference and a hydraulic pressure difference,
determining a set of initial conditions to achieve the desired value, wherein the set of initial conditions comprises one or more of a first reservoir fluid volume, a second reservoir fluid volume, a first concentration of a component in the first reservoir, a second concentration of the component in the second reservoir, and the time dependent imposed pressure difference wherein the set of initial conditions account for a reaction that occurs in the experiment within the microfluidic device, and
initiating the experiment using the set of initial conditions.