CPC G02B 21/0056 (2013.01) [G01J 9/00 (2013.01); G01J 9/0246 (2013.01); G01J 2009/0211 (2013.01); G01J 2009/0269 (2013.01)] | 19 Claims |
9. A quantitative phase image generating device comprising a microscope, the microscope comprising:
an illumination optical system that irradiates illumination light upon an object, the object being a phase body;
an objective lens;
a detector that detects light from the object due to the irradiation of illumination light from the illumination optical system; and
a processor configured to
adjust a position of a focal point of the objective lens so that the focal point of the objective lens is disposed at a plurality of positions that are mutually separated by gaps Δz along an optical axis of the objective lens;
generate three-dimensional light intensity distribution data based upon the light detected at each of the plurality of positions; and
generate a quantitative phase image based upon the three-dimensional light intensity distribution data, wherein:
the quantitative phase image is an image including phases obtained by multiplying change in thickness of the object by change of its refractive index;
the processor sets the gap Δz based upon setting information of the microscope; and
the setting information includes at least one of the following: a numerical aperture NA of the objective lens, a wavelength λ of the illumination light, and a refractive index between the objective lens and the object.
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