US 11,808,770 B2
Proximity assays using chemical ligation and hapten transfer
Christopher Bieniarz, Tucson, AZ (US); and Rui Hong, Oro Valley, AZ (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Mar. 4, 2020, as Appl. No. 16/809,290.
Application 16/809,290 is a division of application No. 15/603,079, filed on May 23, 2017, granted, now 10,620,217.
Application 15/603,079 is a continuation of application No. PCT/EP2015/077484, filed on Nov. 24, 2015.
Claims priority of provisional application 62/116,962, filed on Feb. 17, 2015.
Claims priority of provisional application 62/084,452, filed on Nov. 25, 2014.
Prior Publication US 2020/0271662 A1, Aug. 27, 2020
Int. Cl. G01N 33/68 (2006.01); G01N 33/542 (2006.01); G01N 33/543 (2006.01)
CPC G01N 33/6845 (2013.01) [G01N 33/542 (2013.01); G01N 33/54353 (2013.01); G01N 2440/00 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A composition comprising a first modified binding moiety, wherein the first modified binding moiety comprises:
(a) at least one a cleavable bridge component; and
(b) a first antibody is covalently conjugated to the at least one cleavable bridge component;
wherein the cleavable bridge component has the covalently conjugated structure:
—[cleavage site]—[detectable moiety]—[chemical ligation group], wherein the cleavage site is more proximal to the first antibody than is the detectable moiety and the chemical ligation group, wherein the chemical ligation group is at or near a terminus of the cleavable bridge component, and wherein the chemical ligation group comprises a first reactive functional group; wherein the cleavage site is selected from the group consisting of a disulfide bond, a vicinal diol, 1,4-Bis(2-hydroxymethyl)benzene (“1,4-BHMB”), 1,4-Bis(2-hydroxethyl)benzene (“1,4-BHEB”), 1,3-Bis(2-hydroxymethyl)benzene (“1,3-BHMB”), 2,5-bi s(hydroxymethyl)furan (“2,5-BHMF”), and a vicinal hydroxylamine; wherein the detectable moiety is selected from the group consisting of a peptide tag and an oligonucleotide; and wherein the first reactive functional group is selected from the group consisting of:

OG Complex Work Unit Chemistry

OG Complex Work Unit Chemistry

OG Complex Work Unit Chemistry
RC≡CH,
RC═CH2,
a halogen, and
a pyridine including one R group,
wherein R is H.