	US 11,808,768 B2
	1,1 ′-[[(substituted alkyl)imino]bis (alkylene)]bis- ferrocenes and their use in I electrochemical assays by labelling substrates of interest
Barrie Marsh, Trowbridge (GB); Christopher Frost, Bath And Northeast Somerset (GB); and David Pearce, Trowbridge (GB)
Assigned to Binx Health Limited, Trowbridge (GB)
Filed by Binx Health Limited, Trowbridge (GB)
Filed on Nov. 16, 2020, as Appl. No. 17/099,290.
Application 17/099,290 is a division of application No. 16/664,306, filed on Oct. 25, 2019, granted, now 10,837,967.
Application 16/664,306 is a continuation of application No. 15/906,241, filed on Feb. 27, 2018, granted, now 10,502,744, issued on Dec. 10, 2019.
Application 15/906,241 is a continuation of application No. 14/410,296, abandoned, previously published as PCT/GB2013/051643, filed on Jun. 21, 2013.
Claims priority of application No. 1211157 (GB), filed on Jun. 22, 2012.
Prior Publication US 2021/0116457 A1, Apr. 22, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/58 (2006.01); C07F 17/02 (2006.01); C12Q 1/6816 (2018.01)

CPC G01N 33/58 (2013.01) [C07F 17/02 (2013.01); C12Q 1/6816 (2013.01)]

12 Claims

1. A method of detecting a nucleic acid, the method comprising:

contacting the nucleic acid with a complementary nucleic acid probe under conditions to allow hybridization between said probe and an amplicon; and

selectively degrading the probe, wherein the probe is labelled with a compound selected from the group consisting of:

6-(bis((1′-iodoferrocenyl)1-methylferrocenyl) amino)hexan-1-ol;

6-(bis((1′-(4-nitrophenyl)ferronceyl)1-methylferrocenyl) amino) hexan-1-ol;

6-(bis((1′(1-furanyl)ferrocenyl)1-methylferrocenyl) amino)hexan-1-ol;

6-(bis((1′-chloroferrocenyl)1-methylferrocenyl) amino)hexan-1-ol; and

6-(bis((2-cyano)1-methylferrocenyl) amino)hexan-1-ol.