CPC G01N 33/56972 (2013.01) [C07K 16/00 (2013.01); C07K 16/22 (2013.01); C07K 16/32 (2013.01); G01N 33/50 (2013.01); C07K 2317/10 (2013.01); C07K 2317/92 (2013.01); C12N 2510/02 (2013.01)] | 47 Claims |
1. A method for selecting primary antibody-producing cells that express antibodies exhibiting high affinity binding for an antigen of interest, the method comprising:
(a) contacting a population of primary antibody-producing cells that express antibody to the antigen of interest with a monomeric form of the antigen of interest for a time sufficient for the antigen of interest to bind to antibody on the surface of the cells, wherein the concentration of the monomeric antigen of interest is between 0.1-2 nM or between 5-10 nM;
(b) washing the cells for a period of time to remove unbound antigen;
(c) collecting cells bound to the antigen of interest; and
(d) sorting the cells collected in step (c) to obtain cells that express antibodies of high affinity, wherein the high affinity antibodies have a dissociation equilibrium constant (KD) of 0.1 pM to 25 nM.
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