US 11,808,762 B2
Material and method to screen proteasome stimulators
Darci Jones Trader, West Lafayett, IN (US); and Rachel Anne Coleman, Lafayette, IN (US)
Assigned to Purdue Research Foundation, West Lafayette, IN (US)
Filed by Purdue Research Foundation, West Lafayette, IN (US)
Filed on Aug. 13, 2021, as Appl. No. 17/401,517.
Application 17/401,517 is a division of application No. 16/128,697, filed on Sep. 12, 2018, granted, now 11,092,596.
Claims priority of provisional application 62/559,076, filed on Sep. 15, 2017.
Prior Publication US 2021/0396742 A1, Dec. 23, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 7/08 (2006.01); G01N 33/542 (2006.01); G01N 33/68 (2006.01); G01N 33/573 (2006.01)
CPC G01N 33/542 (2013.01) [C07K 7/08 (2013.01); G01N 33/573 (2013.01); G01N 33/6848 (2013.01); G01N 2333/4706 (2013.01); G01N 2333/9643 (2013.01); G01N 2800/2835 (2013.01)] 2 Claims
 
1. An assay kit for identifying a regulator for core protein 20S (20S CP) comprising a fluorescent reporter and a positive control, wherein said fluorescent reporter having a structure of

OG Complex Work Unit Chemistry
or a salt thereof, wherein CT-L FRET, X=Phe (SEQ ID NO: 1); T-L FRET, X=Arg (SEQ ID NO: 2); and CP-L FRET, X=Asp (SEQ ID NO: 3).