US 11,808,727 B2
Systems and methods for an electrochemical total cholesterol test
Brittney Werner, Beech Grove, IN (US); Aniruddha Patwardhan, Fishers, IN (US); and Gary Hughes, Camby, IN (US)
Assigned to Polymer Technology Systems, Inc., Whitestown, IN (US)
Filed by Polymer Technology Systems, Inc., Whitestown, IN (US)
Filed on Aug. 7, 2017, as Appl. No. 15/670,499.
Claims priority of provisional application 62/529,286, filed on Jul. 6, 2017.
Prior Publication US 2019/0011389 A1, Jan. 10, 2019
Int. Cl. G01N 27/327 (2006.01); C12Q 1/00 (2006.01)
CPC G01N 27/3272 (2013.01) [C12Q 1/005 (2013.01); C12Y 101/03006 (2013.01); G01N 27/3273 (2013.01); G01N 27/3275 (2013.01); G01N 2333/904 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A dry test strip for electrochemical testing of a blood analyte, the test strip comprising:
a first receiving port, the first receiving port for receiving a blood sample, the first receiving port at a first end of the test strip, wherein a blood separation membrane is placed over the first receiving port, the blood separation membrane including lectins;
a first electrode and a second electrode, the first and second electrodes proximate to the first receiving port, including a capillary tube at the first receiving port, wherein the capillary tube leads to the first electrode and the second electrode and in the capillary tube, a dried surfactant solution is located, whereby the dried surfactant solution is wetted by the blood sample;
a first contact and a second contact, the first and second contacts at a second end of the test strip, the first and second contacts interconnected with the first and second electrodes, respectively;
cholesterol oxidase, located proximate to the first and the second electrode;
a mediator, located proximate to the first and the second electrode, wherein the cholesterol oxidase and the mediator interact with the blood sample and the first and second electrode to generate a measurable electrical event, wherein the cholesterol oxidase is configured such that the cholesterol oxidase reacts and directly transfer electrons to the mediator such that mediator interacts with the first and second electrode, wherein the mediator is an osmium compound;
the dried surfactant solution including Sodium Cholate, Potassium Phosphate, and BSA (bovine serum alluvium), located proximate to the first and second electrode.