US 11,808,685 B2
Device for generating light pulses for characterization, standardization or calibration of photodetectors in flow cytometers
Konrad von Volkmann, Berlin (DE); Jan Popien, Berlin (DE); and Frank Reuter, Berlin (DE)
Assigned to APE Angewandte Physik & Elektronik GmbH, Berlin (DE)
Filed by APE Angewandte Physik & Elektronik GmbH, Berlin (DE)
Filed on Nov. 20, 2020, as Appl. No. 17/100,057.
Claims priority of application No. 19210901 (EP), filed on Nov. 22, 2019.
Prior Publication US 2021/0156786 A1, May 27, 2021
Int. Cl. G01N 15/10 (2006.01); H05B 45/32 (2020.01); H05B 45/12 (2020.01); G01N 15/14 (2006.01); G02B 6/42 (2006.01); G01J 1/08 (2006.01); G01J 1/42 (2006.01)
CPC G01N 15/1012 (2013.01) [G01J 1/08 (2013.01); G01N 15/1434 (2013.01); G02B 6/4298 (2013.01); H05B 45/12 (2020.01); H05B 45/32 (2020.01); G01J 1/4257 (2013.01); G01J 2001/4238 (2013.01); G01J 2001/4242 (2013.01); G01J 2001/4252 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A device comprising:
a) a control unit
b) at least one emission light source and
c) a light guide,
wherein the control unit is configured to generate an electrical signal having a predetermined waveform by means of which the at least one emission light source is controlled to generate light pulses from the at least one emission light source, and the at least one emission light source is positioned for coupling the light pulses into the light guide
characterized in that
the device comprises at least one feedback detector and at least one control light source, wherein the at least one control light source is present in series connection with the at least one emission light source, so that the at least one control light source is controlled by means of the same electrical signal for generating light pulses from the at least one emission light source, wherein the electrical signal comprises electrical pulses having a pulse width from 0.1 μs to 100 μs, and a repetition rate from 0.1 to 100 kHz and wherein the at least one control light source is present positioned in such a way that the light pulses from the control light source actually generated by the at least one control light source are not coupled into the light guide, but are detected by the at least one feedback detector and transmitted to a regulating unit which is configured for a readjustment of the electrical signal.