	US 11,808,675 B2
	Room temperature methods for preparing biological analytes
Genevieve Van de Bittner, Campbell, CA (US); James Alexander Apffel, Mountain View, CA (US); Steven M. Fischer, Hayward, CA (US); Christine A. Miller, Campbell, CA (US); and Kristin Briana Bernick, San Jose, CA (US)
Assigned to AGILENT TECHNOLOGIES, INC., Santa Clara, CA (US)
Filed by Agilent Technologies, Inc., Santa Clara, CA (US)
Filed on May 14, 2020, as Appl. No. 16/874,499.
Claims priority of provisional application 62/861,115, filed on Jun. 13, 2019.
Prior Publication US 2020/0393342 A1, Dec. 17, 2020
Int. Cl. G01N 1/28 (2006.01); C12N 1/06 (2006.01); B01D 15/00 (2006.01); C12N 15/10 (2006.01); B01D 11/02 (2006.01); C07K 1/14 (2006.01)

CPC G01N 1/28 (2013.01) [B01D 11/0288 (2013.01); B01D 15/00 (2013.01); C07K 1/145 (2013.01); C12N 1/06 (2013.01); C12N 15/1006 (2013.01)]

22 Claims

1. A method for extracting metabolites from a biological sample, the method comprising:

contacting at room temperature the biological sample with a metabolism-quenching solution comprising a fluoroalcohol,

inhibiting one or more metabolic reactions in the biological sample with the fluoroalcohol;

extracting a mixture from the biological sample with the metabolism-quenching solution comprising the fluoroalcohol, the mixture comprising metabolites, lipids and proteins;

separating the metabolites from the mixture; and

collecting the metabolites.