CPC C40B 50/06 (2013.01) [C12N 15/1079 (2013.01); C12N 15/1093 (2013.01); C12N 15/113 (2013.01); C12Q 1/6876 (2013.01); C40B 50/08 (2013.01); C40B 50/14 (2013.01); G01N 33/502 (2013.01); G01N 33/5029 (2013.01); G01N 33/5041 (2013.01); C12N 2320/30 (2013.01); C12N 2330/31 (2013.01); C12Q 1/6886 (2013.01); C12Q 2600/158 (2013.01)] | 16 Claims |
1. A method for generating a library of variant nucleic acids, the method comprising:
a) providing predetermined nucleic acid sequences, encoding for at least about 30,000 non-identical oligonucleotides, wherein each of the at least about 30,000 non-identical oligonucleotides encodes for a variant codon sequence compared to a single reference sequence, wherein the predetermined nucleic acid sequences encode for 19 variants for each position of multiple codon positions compared to the single reference sequence;
b) providing a structure having a surface;
c) synthesizing the at least about 30,000 non-identical oligonucleotides, wherein each of the at least about 30,000 non-identical oligonucleotides extends from the surface; and
d) mixing the at least about 30,000 non-identical oligonucleotides with a DNA polymerase and the single reference sequence to form a library of variant nucleic acids by amplification.
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