| US 11,807,909 B1 | ||
| Methods for species-level resolution of microorganisms | ||
| Ryan Kemp, Irvine, CA (US); Mike Weinstein, Irvine, CA (US); and Shuiquan Tang, Irvine, CA (US) | ||
| Assigned to ZYMO RESEARCH CORPORATION, Irvine, CA (US) | ||
| Filed by ZYMO RESEARCH CORPORATION, Irvine, CA (US) | ||
| Filed on Jul. 20, 2020, as Appl. No. 16/933,920. | ||
| Claims priority of provisional application 62/899,531, filed on Sep. 12, 2019. | ||
| Int. Cl. C12P 19/34 (2006.01); C12Q 1/689 (2018.01); C12Q 1/6869 (2018.01) | ||
| CPC C12Q 1/689 (2013.01) [C12Q 1/6869 (2013.01)] | 24 Claims |
|
1. A method for the identification and/or quantification of one or more microbes comprising:
(a) extracting DNA from a microbial sample; (b) performing 16S ribosomal RNA gene-targeted sequencing to obtain DNA sequences; and (c) analyzing the DNA sequences to identify the species of the one or more microbes, wherein the sequencing comprises using a pool of V1-V2 primers comprising SEQ ID NOs: 1-11 and/or a pool of V3-V4 primers comprising SEQ ID NOs: 12-19. |