	US 11,807,903 B2
	Methods and compositions for tracking nucleic acid fragment origin for nucleic acid sequencing
Zhoutao Chen, Carlsbad, CA (US); Tsai-Chin Wu, San Marcos, CA (US); Long Kim Pham, San Diego, CA (US); and Yong Wang, Sharon, MA (US)
Assigned to Universal Sequencing Technology Corporation, Canton, MA (US)
Appl. No. 16/968,576
Filed by Universal Sequencing Technology Corporation, Canton, MA (US)
PCT Filed Feb. 8, 2019, PCT No. PCT/US2019/017266 § 371(c)(1), (2) Date Aug. 8, 2020, PCT Pub. No. WO2019/157318, PCT Pub. Date Aug. 15, 2019.
Claims priority of provisional application 62/656,796, filed on Apr. 12, 2018.
Claims priority of provisional application 62/628,079, filed on Feb. 8, 2018.
Prior Publication US 2021/0047683 A1, Feb. 18, 2021
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6804 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6876 (2018.01)

CPC C12Q 1/6869 (2013.01) [C12Q 1/6804 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6876 (2013.01)]

40 Claims

1. A method for tracking nucleic acid fragment origin by barcode tagging comprising:

a. providing a plurality of solid supports having clonal barcode templates or semi-clonal barcode templates immobilized thereon;

b. providing a plurality of transpososomes, each transpososome comprising transposable DNA and transposase, wherein at least one transposable DNA in the transpososome is capable of being captured by said barcode template on said solid support directly or indirectly;

c. providing a nucleic acid target;

d. wherein steps a, b, and c occur in one reaction vessel concurrently to attach a barcode information on said solid support to said nucleic acid target by simultaneous strand transfer and capture reactions without additional partition of each nucleic acid target from another nucleic acid target within the total plurality of nucleic acid targets;

e. breaking said nucleic acid target into fragments by breaking strand transfer complexes, wherein at least one fragment is attached to the barcode template on the solid support.