	US 11,807,899 B2
	Biochemical reaction methods and reagents comprising intrinsically disordered regions
Niall Armes, Cambridge (GB); Hannah Williams, Cambridge (GB); Matthew Forrest, Cambridge (GB); Mathew Parker, Cambridge (GB); Sidong Liu, Cambridge (GB); and Lauren Parker, Cambridge (GB)
Assigned to Biocrucible Limited
Filed by Biocrucible Limited, Cambridge (GB)
Filed on Nov. 19, 2021, as Appl. No. 17/531,349.
Application 17/531,349 is a continuation of application No. PCT/GB2020/052866, filed on Nov. 11, 2020.
Claims priority of application No. 1916379 (GB), filed on Nov. 11, 2019.
Prior Publication US 2022/0145378 A1, May 12, 2022
Int. Cl. C12Q 1/6848 (2018.01); C07K 1/14 (2006.01); C12Q 1/6853 (2018.01)

CPC C12Q 1/6848 (2013.01) [C07K 1/14 (2013.01); C12Q 1/6853 (2013.01); C07K 2319/00 (2013.01)]

26 Claims

1. A method for performing an in-vitro nucleic acid amplification reaction, the method comprising:

performing a recombinase polymerase amplification (RPA) reaction in presence of at least one fusion protein, wherein the at least one fusion protein comprises an RPA component fused to a polypeptide comprising at least one functional intrinsically-disordered region (IDR),

wherein the at least one fusion protein causes liquid-liquid demixing and formation of a plurality of phase-separated aqueous compartments.