CPC C12Q 1/68 (2013.01) [G01N 33/5008 (2013.01); G01N 33/5011 (2013.01); G01N 33/574 (2013.01); C12M 33/00 (2013.01)] | 12 Claims |
1. A method for identifying an agent that inhibits cellular growth and/or viability of tumorigenic or transformed cells in combination with a genetic perturbation, the method comprising:
providing a cellular sample of transformed cells and/or cells obtained from the tumor of a subject, wherein the transformed cells and/or cells obtained from the tumor are adherent cells;
infecting the cells with a barcoded library of lentiviruses encoding one or more CRISPR genetic perturbations, wherein the barcode identifies the one or more CRISPR genetic perturbations;
culturing the cellular sample for at least 5 days under low attachment conditions comprising agitating the cells, wherein the conditions inhibit cell attachment to the culture surface;
contacting or incubating the cellular sample with the agent; and
identifying the one or more genetic perturbations that are depleted in the sample contacted with the agent as compared to a control not contacted with the agent, whereby the agent inhibits cellular growth and/or viability of tumorigenic or transformed cells in combination with the one or more depleted genetic perturbations.
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