	US 11,807,891 B2
	Carbon monoxide and carbon dioxide bioconversion process
Ryan H Senaratne, Fayetteville, AR (US); Nestor A Camargo, Fayetteville, AR (US); Ryan M Lacey, Fayetteville, AR (US); Abel J Price, Fayetteville, AR (US); and Brandon L Beard, Fayetteville, AR (US)
Filed by Jupeng Bio, Inc., League City, TX (US)
Filed on Aug. 2, 2019, as Appl. No. 16/530,502.
Claims priority of provisional application 62/741,797, filed on Oct. 5, 2018.
Claims priority of provisional application 62/741,871, filed on Oct. 5, 2018.
Claims priority of provisional application 62/716,083, filed on Aug. 8, 2018.
Claims priority of provisional application 62/716,071, filed on Aug. 8, 2018.
Claims priority of provisional application 62/716,053, filed on Aug. 8, 2018.
Prior Publication US 2020/0071734 A1, Mar. 5, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/54 (2006.01); C12P 7/52 (2006.01)

CPC C12P 7/54 (2013.01) [C12P 7/52 (2013.01)]

21 Claims

1. A process comprising:

providing a first gaseous substrate to a first bioreactor, the first gaseous substrate comprising CO₂and containing about 5 to about 90 mole % CO₂and about 0 to about 50 mole % CO;

providing a first acetogenic bacteria to the first bioreactor, wherein the first acetogenic bacteria includes a sodium translocating ATPase that is active during fermentation in the first bioreactor;

providing sodium ions to the first bioreactor through one or more sodium ion sources;

fermenting the first gaseous substrate with the first acetogenic bacteria in a fermentation broth comprising the first acetogenic bacteria and the one or more sodium ion sources to produce one or more organic acids, wherein the fermentation broth includes less than about 0.01 grams per liter yeast extract, less than about 0.01 grams per liter carbohydrate, wherein the sodium ions are provided with a sodium feed rate of about 290 to about 8750 μg/gram of cells/minute, and wherein the fermentation broth is maintained at a pH in a range of about 4 to about 6.9, wherein the fermenting of the first gaseous substrate with the first acetogenic bacteria provides about 50 to about 100% conversion of CO₂;

providing at least a portion of the one or more organic acids to a second bioreactor;

providing a second gaseous substrate to the second bioreactor, the second gaseous substrate comprising CO and containing about 5 to about 90 mole % CO;

providing a second acetogenic bacteria to the second bioreactor wherein the second acetogenic bacteria includes a proton translocating ATPase that is active during fermentation in the second bioreactor; and

fermenting the second gaseous substrate in the second bioreactor with the second acetogenic bacteria in a fermentation broth comprising the second acetogenic bacteria to produce a liquid stream comprising one or more alcohols and a gaseous stream comprising CO₂, wherein at least a portion of the gaseous stream is provided to the first bioreactor.