US 11,807,884 B2
Generation-dependent supramolecular assemblies of protein-dendron conjugates
Sandanaraj Selvaraj Britto, Pune (IN); Pavankumar Janardhan Bhandari, Pune (IN); and Mullapudi Mohan Reddy, Pune (IN)
Assigned to INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH, Pune (IN)
Appl. No. 16/771,124
Filed by INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH, Pune (IN)
PCT Filed Dec. 8, 2018, PCT No. PCT/IN2018/050821
§ 371(c)(1), (2) Date Jun. 9, 2020,
PCT Pub. No. WO2019/111282, PCT Pub. Date Jun. 13, 2019.
Claims priority of application No. 201721044279 (IN), filed on Dec. 9, 2017.
Prior Publication US 2021/0163916 A1, Jun. 3, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/96 (2006.01); A61K 47/60 (2017.01); C08G 83/00 (2006.01); C12N 9/64 (2006.01); C12N 9/76 (2006.01); A61K 47/59 (2017.01); B82Y 5/00 (2011.01)
CPC C12N 9/96 (2013.01) [A61K 47/59 (2017.08); A61K 47/60 (2017.08); C08G 83/008 (2013.01); C12N 9/6424 (2013.01); C12N 9/6427 (2013.01); C12Y 304/21001 (2013.01); C12Y 304/21004 (2013.01); C12Y 304/21062 (2013.01); C12Y 304/21064 (2013.01); B82Y 5/00 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A monodisperse protein-dendron conjugate of Formula I:

OG Complex Work Unit Chemistry
wherein:
hydrophilic protein (A) is selected from the group consisting of serine proteases, cysteine proteases, aspartic proteases, metalloproteases, fusion proteins composed of a serine protease and another functional protein, an antibody, and a peptide;
a hydrophobic benzyl ether dendrimer Gn (C) is a dendrimer having formula G2, G3, or G4 represented by:

OG Complex Work Unit Chemistry

OG Complex Work Unit Chemistry
n is 1 to 30;
wherein molecular weight of macromolecular amphiphilic activity-based probe (MAABP) is in a range of 2-8 kDa consisting of a fluorophosphonate of a monodisperse oligoethylene glycol as a hydrophilic spacer (B) and the hydrophobic benzyl ether dendrimer Gn (C).