US 11,807,873 B2
Conversion of S-lignin compounds to useful intermediates
Christopher W. Johnson, Denver, CO (US); Allison Jean Zimont Werner, Denver, CO (US); Gregg Tyler Beckham, Golden, CO (US); and Sandra Fabienne Notonier, Marseilles (FR)
Assigned to Alliance for Sustainable Energy, LLC, Golden, CO (US)
Filed by Alliance for Sustainable Energy, LLC, Golden, CO (US)
Filed on Oct. 19, 2021, as Appl. No. 17/504,901.
Claims priority of provisional application 63/093,636, filed on Oct. 19, 2020.
Prior Publication US 2022/0119849 A1, Apr. 21, 2022
Int. Cl. C12N 9/02 (2006.01); C12P 7/44 (2006.01); C12R 1/39 (2006.01); C12R 1/40 (2006.01); C12N 1/20 (2006.01)
CPC C12N 9/0071 (2013.01) [C12N 9/0069 (2013.01); C12N 9/0073 (2013.01); C12P 7/44 (2013.01); C12N 1/205 (2021.05); C12R 2001/39 (2021.05); C12R 2001/40 (2021.05); C12Y 114/13082 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A genetically modified Pseudomonas sp. comprising:
a first genetic modification of a first gene encoding an exogenous vanillate demethylase, wherein the first gene has a sequence that is at least 80% identical to at least one of SEQ ID NO: 11 or SEQ ID NO: 13, and wherein the expressed exogenous vanillate demethylase has a sequence that is at least 80% identical to at least one of SEQ ID NO: 12 or SEQ ID NO: 14; and
a second genetic modification of a second gene encoding an exogenous protocatechuate dioxygenase, wherein the second gene has a sequence that is at least 80% identical to at least one of SEQ ID NO: 5 or SEQ ID NO: 7, and wherein the exogenous protocatechuate dioxygenase has a sequence that is at least 80% identical to at least one of SEQ ID NO: 6 or SEQ ID NO: 8; wherein:
the Pseudomonas sp. metabolizes a S-lignin decomposition product, and
the Pseudomonas sp. produces 2-pyrone-4,6-dicarboxylate (PDC).