CPC C12N 9/0071 (2013.01) [C12N 9/0069 (2013.01); C12N 9/0073 (2013.01); C12P 7/44 (2013.01); C12N 1/205 (2021.05); C12R 2001/39 (2021.05); C12R 2001/40 (2021.05); C12Y 114/13082 (2013.01)] | 16 Claims |
1. A genetically modified Pseudomonas sp. comprising:
a first genetic modification of a first gene encoding an exogenous vanillate demethylase, wherein the first gene has a sequence that is at least 80% identical to at least one of SEQ ID NO: 11 or SEQ ID NO: 13, and wherein the expressed exogenous vanillate demethylase has a sequence that is at least 80% identical to at least one of SEQ ID NO: 12 or SEQ ID NO: 14; and
a second genetic modification of a second gene encoding an exogenous protocatechuate dioxygenase, wherein the second gene has a sequence that is at least 80% identical to at least one of SEQ ID NO: 5 or SEQ ID NO: 7, and wherein the exogenous protocatechuate dioxygenase has a sequence that is at least 80% identical to at least one of SEQ ID NO: 6 or SEQ ID NO: 8; wherein:
the Pseudomonas sp. metabolizes a S-lignin decomposition product, and
the Pseudomonas sp. produces 2-pyrone-4,6-dicarboxylate (PDC).
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