US 11,807,870 B2
Cellular microcompartment and preparation processes
Maxime Feyeux, Talence (FR); Kevin Alessandri, Bordeaux (FR); Pierre Nassoy, Bordeaux (FR); Laurent Cognet, Bordeaux (FR); Gaëlle Recher, Talence (FR); and Erwan Bezard, Bordeaux (FR)
Assigned to UNIVERSITE DE BORDEAUX, Bordeaux (FR); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE, Palaiseau (FR)
Appl. No. 16/462,962
Filed by UNIVERSITE DE BORDEAUX, Bordeaux (FR); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE, Palaiseau (FR)
PCT Filed Nov. 23, 2017, PCT No. PCT/FR2017/053225
§ 371(c)(1), (2) Date May 22, 2019,
PCT Pub. No. WO2018/096277, PCT Pub. Date May 31, 2018.
Claims priority of application No. 1661377 (FR), filed on Nov. 23, 2016.
Prior Publication US 2019/0330589 A1, Oct. 31, 2019
Int. Cl. C12N 5/00 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12N 5/074 (2010.01)
CPC C12N 5/0075 (2013.01) [C12M 23/20 (2013.01); C12M 25/01 (2013.01); C12M 25/16 (2013.01); C12N 5/0012 (2013.01); C12N 5/0696 (2013.01); C12N 2501/727 (2013.01); C12N 2513/00 (2013.01); C12N 2533/74 (2013.01); C12N 2533/90 (2013.01); C12N 2535/00 (2013.01)] 15 Claims
 
1. A cellular microcompartment, wherein the cellular microcompartment is a cyst comprising a central lumen and the following successively arranged homocentric layers: at least one layer of human pluripotent cells, an extracellular matrix layer, and an outer hydrogel layer, the lumen being hollow and surrounded by human pluripotent cells deposited on or in said extracellular matrix, wherein the human pluripotent cells are polarized and wherein the cell density is between one and several thousand cells per microcompartment, wherein the cellular microcompartment maintains the central lumen after 5 days in culture.