CPC C12N 5/0075 (2013.01) [C12M 23/20 (2013.01); C12M 25/01 (2013.01); C12M 25/16 (2013.01); C12N 5/0012 (2013.01); C12N 5/0696 (2013.01); C12N 2501/727 (2013.01); C12N 2513/00 (2013.01); C12N 2533/74 (2013.01); C12N 2533/90 (2013.01); C12N 2535/00 (2013.01)] | 15 Claims |
1. A cellular microcompartment, wherein the cellular microcompartment is a cyst comprising a central lumen and the following successively arranged homocentric layers: at least one layer of human pluripotent cells, an extracellular matrix layer, and an outer hydrogel layer, the lumen being hollow and surrounded by human pluripotent cells deposited on or in said extracellular matrix, wherein the human pluripotent cells are polarized and wherein the cell density is between one and several thousand cells per microcompartment, wherein the cellular microcompartment maintains the central lumen after 5 days in culture.
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