	US 11,807,869 B2
	Method for producing DNA-edited eukaryotic cell, and kit used in the same
Tomoji Mashimo, Suita (JP); Junji Takeda, Suita (JP); Hiroyuki Morisaka, Suita (JP); and Kazuto Yoshimi, Suita (JP)
Assigned to OSAKA UNIVERSITY, Osaka (JP)
Appl. No. 16/611,308
Filed by OSAKA UNIVERSITY, Suita (JP)
PCT Filed Jun. 8, 2018, PCT No. PCT/JP2018/022066 § 371(c)(1), (2) Date Nov. 6, 2019, PCT Pub. No. WO2018/225858, PCT Pub. Date Dec. 13, 2018.
Claims priority of application No. JP2017-113747 (JP), filed on Jun. 8, 2017.
Prior Publication US 2020/0102580 A1, Apr. 2, 2020
Int. Cl. C12N 1/15 (2006.01); C12N 5/14 (2006.01); C12N 5/16 (2006.01); C12N 9/22 (2006.01); C12N 15/85 (2006.01); A61K 48/00 (2006.01); C12N 15/90 (2006.01)

CPC C12N 15/907 (2013.01) [A61K 48/00 (2013.01); C12N 5/14 (2013.01); C12N 5/16 (2013.01); C12N 9/22 (2013.01); C12N 15/85 (2013.01); C12N 15/8509 (2013.01); C12N 2310/20 (2017.05); C12N 2800/22 (2013.01); C12N 2800/80 (2013.01)]

5 Claims

1. A method for cleaving endogenous DNA in a eukaryotic cell, comprising:

introducing a CRISPR-Cas3 system that can cleave endogenous DNA in a eukaryotic cell, wherein the CRISPR-Cas3 system is a Type I-E or Type I-G CRISPR-Cas3 system, and wherein the CRISPR-Cas3 system includes the following (A) to (C):

(A) a Cas3 protein, a polynucleotide encoding the protein, or an expression vector containing the polynucleotide,

(B) all the Cascade proteins that constitute a Cascade complex, a polynucleotide encoding the Cascade proteins, or an expression vector containing the polynucleotide, and

(C) a pre-crRNA which targets the endogenous DNA, a polynucleotide encoding the pre-crRNA, or an expression vector containing the polynucleotide.