US 11,807,858 B2
Methods for plant transformation using spectinomycin selection
Brian J. Martinell, St. Louis, MO (US); Michael W. Petersen, Sauk City, WI (US); David A. Somers, Mystic, CT (US); Yuechun Wan, Madison, WI (US); Edward Williams, Madison, WI (US); and Xudong Ye, Chesterfield, MO (US)
Assigned to MONSANTO TECHNOLOGY LLC, St. Louis, MO (US)
Filed by Monsanto Technology LLC, St. Louis, MO (US)
Filed on Dec. 10, 2020, as Appl. No. 17/118,539.
Application 15/638,200 is a division of application No. 14/507,744, filed on Oct. 6, 2014, granted, now 9,714,428, issued on Jul. 25, 2017.
Application 14/507,744 is a division of application No. 13/899,452, filed on May 21, 2013, granted, now 8,872,000, issued on Oct. 28, 2014.
Application 13/899,452 is a division of application No. 13/213,021, filed on Aug. 18, 2011, granted, now 8,466,345, issued on Jun. 18, 2013.
Application 13/213,021 is a division of application No. 12/045,562, filed on Mar. 10, 2008, granted, now 8,030,544, issued on Oct. 4, 2011.
Application 17/118,539 is a continuation of application No. 16/393,833, filed on Apr. 24, 2019, granted, now 10,907,167.
Application 16/393,833 is a continuation of application No. 15/638,200, filed on Jun. 29, 2017, abandoned.
Claims priority of provisional application 60/915,066, filed on Apr. 30, 2007.
Claims priority of provisional application 60/894,096, filed on Mar. 9, 2007.
Prior Publication US 2021/0246459 A1, Aug. 12, 2021
Int. Cl. C12N 15/82 (2006.01); C12N 5/00 (2006.01); A01H 4/00 (2006.01); C12N 5/04 (2006.01); G01N 33/00 (2006.01); C12N 9/10 (2006.01); A01H 6/20 (2018.01); A01H 6/54 (2018.01); A01H 6/46 (2018.01); A01H 6/60 (2018.01); C12N 1/20 (2006.01); C12R 1/41 (2006.01)
CPC C12N 15/8209 (2013.01) [A01H 4/003 (2013.01); A01H 4/008 (2013.01); C12N 5/0025 (2013.01); C12N 5/04 (2013.01); C12N 9/1029 (2013.01); C12N 15/8201 (2013.01); C12N 15/8202 (2013.01); C12N 15/8205 (2013.01); C12N 15/8221 (2013.01); C12N 15/8265 (2013.01); C12N 15/8271 (2013.01); C12N 15/8274 (2013.01); C12N 15/8275 (2013.01); C12N 15/8277 (2013.01); C12N 15/8281 (2013.01); G01N 33/0098 (2013.01); A01H 6/202 (2018.05); A01H 6/4684 (2018.05); A01H 6/542 (2018.05); A01H 6/604 (2018.05); C12N 1/205 (2021.05); C12N 15/8261 (2013.01); C12R 2001/41 (2021.05); C12Y 203/01081 (2013.01)] 22 Claims
 
1. A method of producing a transgenic plant comprising:
(a) preparing a dry meristematic explant from a dry plant seed,
(b) pre-culturing the dry meristematic explant prior to transformation in a liquid medium composition for 1-5 days; and
(c) transforming the explant with a heterologous nucleic acid sequence to produce transformed cells comprising the heterologous nucleic acid sequence.