CPC C12N 15/8209 (2013.01) [A01H 4/003 (2013.01); A01H 4/008 (2013.01); C12N 5/0025 (2013.01); C12N 5/04 (2013.01); C12N 9/1029 (2013.01); C12N 15/8201 (2013.01); C12N 15/8202 (2013.01); C12N 15/8205 (2013.01); C12N 15/8221 (2013.01); C12N 15/8265 (2013.01); C12N 15/8271 (2013.01); C12N 15/8274 (2013.01); C12N 15/8275 (2013.01); C12N 15/8277 (2013.01); C12N 15/8281 (2013.01); G01N 33/0098 (2013.01); A01H 6/202 (2018.05); A01H 6/4684 (2018.05); A01H 6/542 (2018.05); A01H 6/604 (2018.05); C12N 1/205 (2021.05); C12N 15/8261 (2013.01); C12R 2001/41 (2021.05); C12Y 203/01081 (2013.01)] | 22 Claims |
1. A method of producing a transgenic plant comprising:
(a) preparing a dry meristematic explant from a dry plant seed,
(b) pre-culturing the dry meristematic explant prior to transformation in a liquid medium composition for 1-5 days; and
(c) transforming the explant with a heterologous nucleic acid sequence to produce transformed cells comprising the heterologous nucleic acid sequence.
|