	US 11,807,857 B2
	Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
Michael Bennett, San Francisco, CA (US); Bill L. Hendrix, West Sacramento, CA (US); Alberto Iandolino, Davis, CA (US); Yao Luo, Davis, CA (US); and Wei Zheng, Davis, CA (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Appl. No. 15/321,969
Filed by Monsanto Technology LLC, St. Louis, MO (US)
PCT Filed Jun. 24, 2015, PCT No. PCT/US2015/037522 § 371(c)(1), (2) Date Dec. 23, 2016, PCT Pub. No. WO2015/200539, PCT Pub. Date Dec. 30, 2015.
Claims priority of provisional application 62/072,888, filed on Oct. 30, 2014.
Claims priority of provisional application 62/017,196, filed on Jun. 25, 2014.
Prior Publication US 2017/0130237 A1, May 11, 2017
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8206 (2013.01) [C12N 15/8218 (2013.01); C12N 15/8274 (2013.01); C12N 15/8281 (2013.01); C12N 15/8282 (2013.01); C12N 15/8283 (2013.01); C12N 15/8285 (2013.01); C12N 15/8286 (2013.01)]

12 Claims

1. A method for delivering one or more polynucleotides into a plant cell, comprising applying onto the intact surface of a plant or an intact part thereof a mixture comprising:

a) a cationic polyelectrolyte;

b) an osmolyte; and

c) the one or more polynucleotides,

wherein the cationic polyelectrolyte is hexadimethrine bromide,

wherein the osmolyte comprises a carbohydrate or a sugar alcohol, and

wherein the one or more polynucleotides comprise at least one segment of 18 or more contiguous nucleotides that shares about 90% to about 100% sequence identity to a fragment of a target gene, or the complement thereof.