US 11,807,842 B2
Fluidic array systems and testing for cells, organoids, and organ cultures
John Collins, Irvine, CA (US)
Assigned to Biopico Systems Inc, Irvine, CA (US)
Filed by John Collins, Irvine, CA (US)
Filed on Sep. 30, 2020, as Appl. No. 16/948,734.
Claims priority of provisional application 62/908,201, filed on Sep. 30, 2019.
Prior Publication US 2021/0095235 A1, Apr. 1, 2021
Int. Cl. C12M 3/00 (2006.01); B01L 3/00 (2006.01); C12M 3/06 (2006.01); C12M 1/32 (2006.01)
CPC C12M 23/16 (2013.01) [B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502746 (2013.01); B01L 3/502761 (2013.01); C12M 23/12 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0848 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/086 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for a culture system to model fluid flow for maturing organs in a human physiological system, the method comprising:
using a fluidic plate with a plurality of wells; wherein the plurality of wells are connected by at least one of a plurality of fluidic channels;
inserting a fluid into at least one of the plurality of wells; and
tilting the fluidic plate to create flow in the fluid within the at least one of the plurality of wells; wherein tilting the fluidic plate so that each corner of the fluidic plate raises and lowers individually; wherein the tilting creates unidirectional flow through the plurality of wells connected by the plurality of fluidic channels;
wherein a unidirectional fluidic recirculation loop is formed in the fluidic channels connected between at least three wells of the plurality of wells caused by the tilting;
wherein the fluid in the culture system comprises at least one of a plurality of cells, organs, organoids, and cell media.