CPC C12M 23/16 (2013.01) [B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502746 (2013.01); B01L 3/502761 (2013.01); C12M 23/12 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0848 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/086 (2013.01)] | 16 Claims |
1. A method for a culture system to model fluid flow for maturing organs in a human physiological system, the method comprising:
using a fluidic plate with a plurality of wells; wherein the plurality of wells are connected by at least one of a plurality of fluidic channels;
inserting a fluid into at least one of the plurality of wells; and
tilting the fluidic plate to create flow in the fluid within the at least one of the plurality of wells; wherein tilting the fluidic plate so that each corner of the fluidic plate raises and lowers individually; wherein the tilting creates unidirectional flow through the plurality of wells connected by the plurality of fluidic channels;
wherein a unidirectional fluidic recirculation loop is formed in the fluidic channels connected between at least three wells of the plurality of wells caused by the tilting;
wherein the fluid in the culture system comprises at least one of a plurality of cells, organs, organoids, and cell media.
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