US 11,807,684 B2
Humanized and chimeric monoclonal antibodies to CD47
Jie Liu, Palo Alto, CA (US); Irving L. Weissman, Stanford, CA (US); and Ravindra Majeti, Palo Alto, CA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed on Apr. 21, 2021, as Appl. No. 17/236,810.
Application 14/656,431 is a division of application No. 13/675,274, filed on Nov. 13, 2012, granted, now 9,017,675, issued on Apr. 28, 2015.
Application 17/236,810 is a continuation of application No. 17/108,731, filed on Dec. 1, 2020, granted, now 11,014,985.
Application 17/108,731 is a continuation of application No. 15/175,848, filed on Jun. 7, 2016, abandoned.
Application 15/175,848 is a continuation of application No. 14/656,431, filed on Mar. 12, 2015, granted, now 9,382,320, issued on Jul. 5, 2016.
Application 13/675,274 is a continuation in part of application No. PCT/US2011/036535, filed on May 13, 2011.
Claims priority of provisional application 61/395,652, filed on May 14, 2010.
Prior Publication US 2021/0246206 A1, Aug. 12, 2021
Int. Cl. G01N 33/53 (2006.01); C07K 16/28 (2006.01); G01N 33/68 (2006.01); C07K 16/46 (2006.01)
CPC C07K 16/2803 (2013.01) [C07K 16/2896 (2013.01); C07K 16/465 (2013.01); G01N 33/6872 (2013.01); C07K 2317/24 (2013.01); C07K 2317/56 (2013.01); C07K 2317/565 (2013.01); C07K 2317/567 (2013.01); C07K 2317/70 (2013.01); C07K 2317/71 (2013.01); C07K 2317/73 (2013.01); C07K 2317/732 (2013.01); C07K 2317/734 (2013.01); C07K 2317/76 (2013.01); C07K 2317/94 (2013.01)] 9 Claims
 
1. A method of detecting the presence of CD47 in a biological sample or tissue, the method comprising:
contacting said sample or tissues with an antibody that specifically binds to human CD47, wherein said light chain comprises each of the CDR sequences set forth in SEQ ID NO:23-25; SEQ ID NO:31-33, or SEQ ID NO:64-66; and wherein said heavy chain comprises each of the CDR sequences set forth in SEQ ID NO:20-22 or SEQ ID NO:28-30 or SEQ ID NO:61-63.