CPC C07K 1/18 (2013.01) [A61K 35/16 (2013.01); A61K 38/36 (2013.01); A61K 38/4846 (2013.01); B01D 15/34 (2013.01); B01D 15/363 (2013.01); B01D 15/3847 (2013.01); B01J 41/20 (2013.01); C07K 14/472 (2013.01); C07K 14/755 (2013.01); C07K 14/765 (2013.01); C07K 16/00 (2013.01); C12N 9/644 (2013.01); C12N 9/6424 (2013.01); C12Y 304/21022 (2013.01); A61K 38/00 (2013.01)] | 16 Claims |
1. A chromatographic method comprising the following steps:
loading proteins on a chromatography column packed with a resin comprising porous lid beads having an inner porous core and an outer porous lid, wherein the inner core is provided with anion exchange ligands, and wherein the porosity of the lid and core does not allow entering of molecules larger than 500 kD;
adsorbing Factor IX (FIX) on the anion exchange ligands in the core;
collecting separated proteins in the flow through; and
eluting FIX from the ligands in the core.
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