US 11,806,719 B2
Integrated solid-state rapid thermo-cycling system
N. Reginald Beer, Pleasanton, CA (US); and Gary Johnson, Livermore, CA (US)
Assigned to Lawrence Livermore National Security, LLC, Livermore, CA (US)
Filed by Lawrence Livermore National Security, LLC, Livermore, CA (US)
Filed on Aug. 2, 2022, as Appl. No. 17/816,886.
Application 17/816,886 is a division of application No. 16/058,953, filed on Aug. 8, 2018, granted, now 11,440,015.
Prior Publication US 2023/0166265 A1, Jun. 1, 2023
Int. Cl. B01L 7/00 (2006.01); B01L 99/00 (2010.01); C12Q 1/6869 (2018.01); H10N 10/13 (2023.01)
CPC B01L 7/525 (2013.01) [C12Q 1/6869 (2013.01); H10N 10/13 (2023.02); B01L 2300/1805 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method of operating an integrated thermal cycling system, comprising:
depositing samples into the integrated thermal cycling system, wherein the integrated thermal cycling system comprises a thermal cycling device and an electronic interface, wherein the thermal cycling device comprises: multiple wells to receive the samples to be thermally cycled, a thermoelectric cooling (TEC) element connected to the multiple wells, a substrate on which the TEC element is positioned, and a controller coupled to the TEC element to control operation of the TEC element, wherein the multiple wells are positioned within the substrate, and wherein the substrate comprises a thermally conductive ground positioned between adjacent wells;
coupling the integrated thermal cycling system to a computing device via the electronic interface;
supplying power to the integrated thermal cycling system, via the electronic interface, to allow the multiple wells to exchange heat with the substrate and to allow each well of the multiple wells to operate independently from one another;
wherein supplying of the power to the integrated circuit comprises: transferring energy from a first well to a second well of the multiple wells when current flows through the TEC element in a first direction, and from the second well to the first well of the multiple wells when current flows through the TEC element in a second direction; and
collecting data from the controller of the integrated thermal cycling system via a data line.