US 11,806,462 B2
Triple syringe and methods of making platelet-enriched plasma and use thereof
Christopher Bare, Naples, FL (US)
Assigned to Arthrex, Inc., Naples, FL (US)
Filed by ARTHREX, INC., Naples, FL (US)
Filed on Apr. 6, 2021, as Appl. No. 17/223,277.
Application 17/223,277 is a continuation of application No. 14/605,657, filed on Jan. 26, 2015, abandoned.
Claims priority of provisional application 61/931,956, filed on Jan. 27, 2014.
Prior Publication US 2021/0220543 A1, Jul. 22, 2021
Int. Cl. G01N 1/40 (2006.01); A61M 1/00 (2006.01); A61M 1/38 (2006.01); A61M 1/02 (2006.01); A61B 5/15 (2006.01); A61B 5/153 (2006.01); A61M 5/19 (2006.01); C12N 5/078 (2010.01)
CPC A61M 1/382 (2013.01) [A61B 5/15003 (2013.01); A61B 5/153 (2013.01); A61B 5/150236 (2013.01); A61B 5/150244 (2013.01); A61B 5/150251 (2013.01); A61B 5/150755 (2013.01); A61M 1/029 (2013.01); A61M 5/19 (2013.01); G01N 1/4077 (2013.01); A61M 2202/0415 (2013.01); A61M 2202/10 (2013.01); C12N 5/0634 (2013.01); C12N 5/0641 (2013.01); Y10T 436/25375 (2015.01)] 18 Claims
OG exemplary drawing
 
1. A method of obtaining platelet enriched plasma, comprising:
(a) introducing blood or bone marrow aspirate into a first body of a first syringe of a nested set of syringes, wherein the nested set of syringes comprises the first syringe having the first body with a first diameter; a second syringe having a second body with a second diameter smaller than the first diameter; and a third syringe having a third body with a third diameter smaller than the second diameter; wherein the first syringe is in direct fluid communication with the second syringe and the third syringe, wherein the second syringe body is located partially within the body of the first syringe, and wherein the third syringe body is located partially within the body of the second syringe;
(c) subjecting the nested set of syringes to centrifugation to obtain a plurality of blood or bone marrow fractions including a first fraction, a second fraction, and a third fraction; and
(d) transferring the first fraction of the plurality of fractions from the first syringe to the third syringe; and transferring the second fraction of the plurality of fractions from the first syringe to the second syringe.