US 11,806,432 B2
Compositions, methods and uses for thermally stable multi-targeted antigens
Theodore Randolph, Niwot, CO (US); and Robert Garcea, Boulder, CO (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE, Denver, CO (US)
Filed by THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE, Denver, CO (US)
Filed on Feb. 4, 2022, as Appl. No. 17/665,285.
Application 17/665,285 is a continuation of application No. 16/146,686, filed on Sep. 28, 2018, granted, now 11,273,127.
Application 16/146,686 is a continuation in part of application No. 15/309,169, abandoned, previously published as PCT/US2015/029529, filed on May 6, 2015.
Claims priority of provisional application 61/989,365, filed on May 6, 2014.
Prior Publication US 2022/0175907 A1, Jun. 9, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 9/16 (2006.01); A61K 9/19 (2006.01); C12N 7/00 (2006.01); A61K 39/12 (2006.01); A61K 39/295 (2006.01); A61K 45/06 (2006.01); A61K 39/00 (2006.01)
CPC A61K 9/19 (2013.01) [A61K 9/1623 (2013.01); A61K 9/1694 (2013.01); A61K 39/12 (2013.01); A61K 39/295 (2013.01); A61K 45/06 (2013.01); C12N 7/00 (2013.01); A61K 2039/5252 (2013.01); A61K 2039/5258 (2013.01); A61K 2039/55505 (2013.01); A61K 2039/55572 (2013.01); C12N 2710/20034 (2013.01); C12N 2710/20063 (2013.01); C12N 2710/20071 (2013.01)] 25 Claims
 
1. An essentially dried immunogenic composition comprising:
a single broad-spectrum multi-targeted antigen complex of two or more different pathogens or serotypes, wherein the single complex comprises at least two assembled subunits from the two or more different pathogens or serotypes;
one or more aluminum salt adjuvants;
one or more immunologically-related co-stimulatory agents;
one or more non-reducing disaccharide agents selected from the group consisting of trehalose, sucrose, or a combination thereof;
one or more volatile salts, wherein the one or more volatile salts are selected from the group consisting of one or more of ammonium acetate, ammonium formate, triethylammonium acetate, triethylammonium formate, triethylammonium carbonate, trimethylamine acetate, trimethylamine formate, trimethylamine carbonate, pyridinal acetate and pyridinal formate, or combinations thereof; and
wherein the essentially dried immunogenic composition excludes mannitol.