US 11,806,370 B2
Methods of preparing and using placental tissue compositions
Scott A. Washburn, Pfafftown, NC (US); and Seth Tomblyn, Winston-Salem, NC (US)
Assigned to Plakous Therapeutics, Inc., Winston-Salem, NC (US)
Filed by Plakous Therapeutics, Inc., Winston-Salem, NC (US)
Filed on Mar. 26, 2021, as Appl. No. 17/214,144.
Application 17/214,144 is a division of application No. 16/480,395, granted, now 10,960,028, previously published as PCT/US2018/016383, filed on Feb. 1, 2018.
Claims priority of provisional application 62/453,335, filed on Feb. 1, 2017.
Prior Publication US 2021/0213070 A1, Jul. 15, 2021
Int. Cl. A61K 35/50 (2015.01); A61P 17/02 (2006.01); A61K 9/70 (2006.01)
CPC A61K 35/50 (2013.01) [A61K 9/7007 (2013.01); A61P 17/02 (2018.01)] 14 Claims
OG exemplary drawing
 
1. A method of preparing a composition for treatment of a wound or lesion in a subject in need thereof, comprising:
(a) contacting an ex-vivo amnion comprising a collagen-containing layer with an amount of a processing agent effective for expanding the collagen-containing layer; and
(b) expanding the collagen-containing layer of the ex-vivo amnion, wherein the expanded collagen-containing layer increases a capacity of the ex-vivo amnion to receive a therapeutic agent for treatment of the wound or lesion in the subject.