	US 11,805,805 B2
	Purification of tobacco-derived protein compositions
Kyle Ford, Germanton, NC (US); John-Paul Mua, Advance, NC (US); Nicholas Partain, Owensboro, KY (US); Joshua D. Morton, Evansville, IN (US); and Barry Bratcher, Owensboro, KY (US)
Assigned to R.J. Reynolds Tobacco Company, Winston-Salem, NC (US)
Filed by R.J. Reynolds Tobacco Company, Winston-Salem, NC (US)
Filed on Jul. 28, 2020, as Appl. No. 16/940,698.
Application 16/940,698 is a division of application No. 15/654,834, filed on Jul. 20, 2017, granted, now 10,757,964.
Prior Publication US 2020/0352213 A1, Nov. 12, 2020
Int. Cl. A61K 36/81 (2006.01); A24B 15/24 (2006.01); A23J 3/14 (2006.01); A23J 1/00 (2006.01); A24B 15/28 (2006.01); A24B 15/30 (2006.01); C07K 1/14 (2006.01); C07K 14/415 (2006.01)

CPC A24B 15/241 (2013.01) [A23J 1/007 (2013.01); A23J 3/14 (2013.01); A24B 15/28 (2013.01); A24B 15/303 (2013.01); C07K 1/145 (2013.01); C07K 14/415 (2013.01); C12Y 401/01039 (2013.01)]

7 Claims

1. A method for reducing the nicotine content in a plant-derived protein-enriched material, the method comprising:

a) receiving a plant-derived, protein-enriched material comprising RuBisCO, F2 fraction proteins, or a combination thereof, wherein the plant-derived, protein-enriched material further comprises undesirable nicotine;

b) treating the plant-derived, protein-enriched material with a basic solution to give a base-treated mixture;

c) mixing the base-treated mixture with hydrogen peroxide to give a hydrogen-peroxide-treated material; and

d) processing the basic solution on a filter to afford a retentate comprising a protein concentrate or isolate having a reduced amount of nicotine as compared with the plant-derived, protein-enriched material.