US 11,805,790 B2
Zymolytic rice protein powder with low granular sensation, methods for preparing and application thereof
Tianxiang Chen, Wuxi (CN); Qiusheng Yu, Wuxi (CN); and Xiangli Ping, Wuxi (CN)
Assigned to WUXI JINNONG BIOTECHNOLOGY CO., LTD, Wuxi (CN)
Appl. No. 16/962,843
Filed by WUXI JINNONG BIOTECHNOLOGY CO., LTD, Wuxi (CN)
PCT Filed Jul. 30, 2019, PCT No. PCT/CN2019/098291
§ 371(c)(1), (2) Date Jul. 17, 2020,
PCT Pub. No. WO2020/113973, PCT Pub. Date Jun. 11, 2020.
Claims priority of application No. 201811462973.1 (CN), filed on Dec. 3, 2018.
Prior Publication US 2021/0127707 A1, May 6, 2021
Int. Cl. A23J 1/12 (2006.01); A23K 10/30 (2016.01); A23L 33/185 (2016.01); A23J 3/14 (2006.01); A23L 2/66 (2006.01)
CPC A23J 1/12 (2013.01) [A23J 3/14 (2013.01); A23K 10/30 (2016.05); A23L 2/66 (2013.01); A23L 33/185 (2016.08)] 6 Claims
 
1. A method for preparing a zymolytic rice protein powder with low granular sensation, the zymolytic rice protein powder comprises soluble proteins, wherein more than 70% of the soluble proteins has a molecular weight less than 2000 Da, the method comprising:
(1) pretreatment of a rice residue after sugar production: using the rice residue as a first raw material, and sieving by 40 mesh so as to remove large particular impurities to obtain a second raw material;
(2) high temperature extrusion pretreatment: mixing the second raw material obtained in step (1) with water with a water content of 10-20 wt. %;
performing the extrusion pretreatment in an extruder at a temperature of 130-160° C. to obtain a rice residue solution;
(3) refining treatment: adjusting the pH of the rice residue solution to 7.2-8.0 by an alkali solution; separating the rice residue solution by a solid-liquid separation to obtain a-wet protein residue with more than 80% protein content;
(4) wet crushing: adjusting the wet protein residue to a concentration of 10-30 wt. %; performing a wet classification and a first grinding treatment to obtain a protein material solution; wherein particles of the protein material solution are approximately 100 mesh; separating by a pressure arc sieve to obtain an upper fraction of the protein material solution and a lower fraction of the protein material solution, wherein the upper fraction of the protein material solution is crushed by a second grinding treatment and the lower fraction of the protein material solution is subjected to zymolysis;
(5) zymolysis control: standardizing the lower fraction of the protein material solution obtained in step (4); adjusting the temperature of the protein material solution to 45-55° C., adjusting the pH of the protein material solution to 5.5-8.5, and performing a partial protein hydrolysis on the protein material solution by a synergistic action of compound endopeptidases to obtain a protein hydrolysate;
(6) zymolysis termination: keeping the protein hydrolysate at 80-90° C. for 30 minutes so as to stop the zymolysis;
(7) microjet treatment: delivering the protein hydrolysate into a microjet with a pressure of 30-35 MPa, and repeating for 2 to 4 times to obtain a first processed material, wherein the particle size of the first processed material is from 10 to 25 μm;
(8) spray drying: drying the first processed material by spray drying with the air inlet temperature ranging from 180-200° C. and the air outlet temperature ranging from 80-100° C. to obtain a sample; wherein the water content of the sample is 5% after the spray drying;
(9) crushing: crushing the sample after the spray drying, wherein the sample is configured to prepare products comprising soluble proteins, wherein more than 70% of the soluble proteins having a molecular weight less than 2000 Da.