US 11,805,775 B2
Perfusion bioreactor, perfusion device, artificial liver system, and related methods
Radu Alexandru Paun, L'Ile Perrot (CA); Shawana Habib, Montreal (CA); Massimiliano Paganelli, Outremont (CA); and Claudia Raggi, Outremont (CA)
Assigned to MORPHOCELL TECHNOLOGIES INC., Montreal (CA)
Filed by MORPHOCELL TECHNOLOGIES INC., Montreal (CA)
Filed on Jul. 16, 2021, as Appl. No. 17/377,574.
Application 17/377,574 is a continuation of application No. 17/057,061, granted, now 11,096,388, previously published as PCT/CA2019/050698, filed on May 22, 2019.
Claims priority of provisional application 62/674,696, filed on May 22, 2018.
Prior Publication US 2021/0337783 A1, Nov. 4, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A01N 1/02 (2006.01); C12M 3/00 (2006.01); C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01); C12N 5/071 (2010.01)
CPC A01N 1/0247 (2013.01) [A01N 1/021 (2013.01); C12M 21/08 (2013.01); C12M 25/02 (2013.01); C12M 29/04 (2013.01); C12M 29/10 (2013.01); C12M 29/24 (2013.01); C12M 33/14 (2013.01); C12N 5/0697 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A perfusion bioreactor, comprising:
a housing having a length defined between a housing inlet and a housing outlet, the housing having an inner surface delimiting an internal cavity of the housing disposed between the housing inlet and the housing outlet and in fluid communication therewith; and
perfusion devices disposed in the internal cavity of the housing, each of the perfusion devices comprising:
a mesh structure supported from the inner surface of the housing, the mesh structure having a first wall spaced apart from a second wall to define an internal mesh cavity, each of the first and second walls of the mesh structure having openings therein to permit fluid communication through the mesh structure; and
an encapsulated organ tissue disposed in the internal mesh cavity between the first and second walls of the mesh structure, the encapsulated organ tissue having at least one organoid at least partially covered with a biocompatible cross-linked polymer, the encapsulated organ tissue having a body with a thickness defined between a first surface of the body adjacent the first wall of the mesh structure and a second surface of the body adjacent the second wall of the mesh structure, the body having at least one channel extending into the body from one of the first and second surfaces to receive a fluid therein, the at least one channel having a diameter selected to diffuse solutes out of the fluid and into the body;
the perfusion devices being disposed in the internal cavity of the housing one adjacent to another and spaced apart from each other along the length of the housing to receive the fluid conveyed from the housing inlet to the housing outlet, and to perfuse the fluid to the encapsulated organ tissue of each perfusion device and to the at least one channel therein.