	US 11,791,017 B2
	Soybean anti-pod-shattering major QTLqPD05, and mapping method and application thereof
Lijuan Qiu, Beijing (CN); Dezhi Han, Beijing (CN); Jianan Han, Beijing (CN); Hongrui Yan, Beijing (CN); and Bohong Su, Beijing (CN)
Assigned to Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing (CN)
Filed by INSTITUTE OF CROP SCIENCES, CHINESE ACADEMY OF AGRICULTURAL SCIENCES, Beijing (CN)
Filed on Apr. 23, 2019, as Appl. No. 16/392,053.
Claims priority of application No. 201810375333.0 (CN), filed on Apr. 24, 2018.
Prior Publication US 2019/0333602 A1, Oct. 31, 2019
Int. Cl. G16B 20/40 (2019.01); G16B 20/20 (2019.01); G16B 40/10 (2019.01); C12Q 1/6895 (2018.01); C12Q 1/6897 (2018.01); C12Q 1/6869 (2018.01)

CPC G16B 20/40 (2019.02) [C12Q 1/6895 (2013.01); C12Q 1/6897 (2013.01); G16B 20/20 (2019.02); G16B 40/10 (2019.02); C12Q 1/6869 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/158 (2013.01)]

8 Claims

1. A method for identifying a genotype of recombinant inbred lines (RILs) in soybeans with pod-shattering trait, comprising steps of:

(1) mapping soybean anti-pod-shattering major quantitative trait locus (QTL) using two soybean parent plants and resulting RILs;

(2) performing PCR amplification using genomic DNAs of the two soybean parent plants and the resulting RILs as templates and with

a forward primer comprises a nucleotide sequence as shown in SEQ ID NO. 1 and

a reverse primer comprises a nucleotide sequence as shown in SEQ ID NO. 2;

(3) digesting PCR fragments generated in step (2) with HpaII to produce digested fragments; and

(4) determining the genotype of RILs based on presence of target digested fragments in the digested fragments in step (3);

wherein the major QTL in step (1) is mapped on chromosome 5 of the soybeans.