CPC G01N 33/56983 (2013.01) [G01N 33/54346 (2013.01); G01N 33/54388 (2021.08); G01N 2333/165 (2013.01); G01N 2469/20 (2013.01); G01N 2800/26 (2013.01)] | 54 Claims |
1. A method for detection and measurement of neutralizing antibody levels to SARS-COV-2 in a test-specimen, said method comprising:
obtaining a test-specimen from a subject;
transferring the test-specimen to a sample well of a test-cassette, wherein the cassette further comprises a sample pad, a conjugate pad, a nitrocellulose membrane and an absorbent pad, wherein the sample pad and/or conjugate pad comprises ACE2 or a functional fragment thereof prior to introduction of a sample, wherein the conjugate pad comprises a viral-ACE2-binding protein coupled to a label prior to introduction of a sample;
adding a buffer; and
reading the results from the test-cassette.
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