CPC G01N 33/54326 (2013.01) [B01F 31/65 (2022.01); B01F 33/3021 (2022.01); B01F 33/3031 (2022.01); B01F 33/451 (2022.01); B01L 3/50273 (2013.01); B01L 3/502761 (2013.01); B01L 3/502792 (2013.01); G01N 1/38 (2013.01); B01L 7/52 (2013.01); B01L 7/525 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/089 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0424 (2013.01); B01L 2400/0427 (2013.01); B01L 2400/0436 (2013.01); B01L 2400/0442 (2013.01); G01N 2001/386 (2013.01); G01N 2035/1046 (2013.01); Y10T 436/11 (2015.01); Y10T 436/25 (2015.01); Y10T 436/2575 (2015.01); Y10T 436/25625 (2015.01)] | 9 Claims |
1. A method comprising:
substantially immobilizing magnetically responsive beads within a droplet using a magnetic field; and
using a plurality of droplet operations electrodes to split the droplet into first and second droplets, wherein the first droplet contains at least substantially all of the magnetically responsive beads and the second droplet is at least substantially lacking in magnetically responsive beads, further comprising using a physical barrier to facilitate splitting of the droplet, where the physical barrier reduces a gap distance at a point of splitting.
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