CPC G01N 33/5067 (2013.01) [B01L 7/00 (2013.01); C12M 25/00 (2013.01); C12M 29/00 (2013.01); C12M 41/46 (2013.01); C12M 47/20 (2013.01); G01N 33/15 (2013.01); G01N 33/502 (2013.01); G01N 35/1009 (2013.01); B01L 3/5085 (2013.01); B01L 9/543 (2013.01); G01N 2035/00425 (2013.01); G01N 2035/0418 (2013.01)] | 13 Claims |
6. A componential analysis method for evaluating an amount of a chemical substance which is taken up into a cell or excreted from a cell, the method comprising:
a first step of adding a first buffer solution to a container which comprises a cell containing a chemical substance to be evaluated, adjusting a temperature within the container to a first temperature and incubating, and adjusting the temperature within the container to a second temperature which is lower than the first temperature;
a second step of collecting the first buffer solution from the container after the first step;
a third step of adding a second buffer solution to the container after the second step, and adjusting the temperature within the container to a third temperature and incubating;
a first determination step of determining an amount S of the chemical substance which is excreted from the cell to the first buffer solution in the container after the first step;
a second determination step of determining an amount B2 of the chemical substance which is excreted from the cell to the second buffer solution in the container after the third step; and
evaluating an amount of the chemical substance excreted from a basal/basolateral side of the cell based on the amount S of the chemical substance.
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