US 11,789,013 B2
Componential analyzer, drug efficacy analyzer, and analysis method
Ryosuke Takahashi, Tokyo (JP); Katsuhiro Kanda, Tokyo (JP); Yusuke Shimizu, Tokyo (JP); and Hisashi Ichikawa, Tokyo (JP)
Assigned to Hitachi High-Tech Corporation, Tokyo (JP)
Filed by Hitachi High-Tech Corporation, Tokyo (JP)
Filed on Apr. 23, 2020, as Appl. No. 16/856,459.
Application 16/856,459 is a continuation of application No. 15/847,092, filed on Dec. 19, 2017, granted, now 10,684,276.
Application 15/847,092 is a continuation of application No. 14/914,278, granted, now 9,880,153, issued on Jan. 30, 2018, previously published as PCT/JP2014/081135, filed on Nov. 26, 2014.
Claims priority of application No. 2013-246906 (JP), filed on Nov. 29, 2013.
Prior Publication US 2020/0249223 A1, Aug. 6, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/50 (2006.01); G01N 35/10 (2006.01); G01N 33/15 (2006.01); G01N 35/00 (2006.01); G01N 35/04 (2006.01); B01L 7/00 (2006.01); C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); B01L 3/00 (2006.01); B01L 9/00 (2006.01)
CPC G01N 33/5067 (2013.01) [B01L 7/00 (2013.01); C12M 25/00 (2013.01); C12M 29/00 (2013.01); C12M 41/46 (2013.01); C12M 47/20 (2013.01); G01N 33/15 (2013.01); G01N 33/502 (2013.01); G01N 35/1009 (2013.01); B01L 3/5085 (2013.01); B01L 9/543 (2013.01); G01N 2035/00425 (2013.01); G01N 2035/0418 (2013.01)] 13 Claims
OG exemplary drawing
 
6. A componential analysis method for evaluating an amount of a chemical substance which is taken up into a cell or excreted from a cell, the method comprising:
a first step of adding a first buffer solution to a container which comprises a cell containing a chemical substance to be evaluated, adjusting a temperature within the container to a first temperature and incubating, and adjusting the temperature within the container to a second temperature which is lower than the first temperature;
a second step of collecting the first buffer solution from the container after the first step;
a third step of adding a second buffer solution to the container after the second step, and adjusting the temperature within the container to a third temperature and incubating;
a first determination step of determining an amount S of the chemical substance which is excreted from the cell to the first buffer solution in the container after the first step;
a second determination step of determining an amount B2 of the chemical substance which is excreted from the cell to the second buffer solution in the container after the third step; and
evaluating an amount of the chemical substance excreted from a basal/basolateral side of the cell based on the amount S of the chemical substance.